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pSP64TS porcine TGFbeta1 (DM#167)
(Plasmid #15011)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15011 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSP64TS
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Sp6 expression for use in Xenopus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TGFb type 1
  • Alt name
    TGFB1
  • Alt name
    TGF beta type I
  • Species
    porcine (Sus scrofa)
  • Insert Size (bp)
    1200
  • Entrez Gene
    TGFB1 (a.k.a. TGF-BETA-1)
  • Tag / Fusion Protein
    • Flag / HMK

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The FLAG epitope (DYKDDDK) and a protein kinase recognition site (RRASV) were inserted three amino acids downstream of the tetrabasic cleavage site.

See Author's Map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP64TS porcine TGFbeta1 (DM#167) was a gift from Douglas Melton (Addgene plasmid # 15011 ; http://n2t.net/addgene:15011 ; RRID:Addgene_15011)
  • For your References section:

    Short-range signaling by candidate morphogens of the TGF beta family and evidence for a relay mechanism of induction. Reilly KM, Melton DA. Cell. 1996 Sep 6. 86(5):743-54. 10.1016/S0092-8674(00)80149-X PubMed 8797821