pRAVE-nLacZ (CC#111)
(Plasmid
#15135)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15135 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepRAVE
-
Backbone manufacturerCepko Lab
-
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namenLacZ
-
Alt namenuclear LacZ
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
In addition to the long terminal repeat (LTR) promoter, the RAVE-nlacZ viral
vector has an internal Xenopus elongation factor alpha (EF1-alpha) promoter. To construct RAVE-nlacZ, the Xenopus EF1-alpha gene from pEF-puro (from Gord Fishell), was cloned into the pRIA vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRAVE-nLacZ (CC#111) was a gift from Connie Cepko (Addgene plasmid # 15135 ; http://n2t.net/addgene:15135 ; RRID:Addgene_15135) -
For your References section:
The dorsal-ventral axis of the neural retina is divided into multiple domains of restricted gene expression which exhibit features of lineage compartments. Peters MA, Cepko CL. Dev Biol. 2002 Nov 1. 251(1):59-73. 10.1006/dbio.2002.0791 PubMed 12413898