pMAH-EcaYRS
(Plasmid #153558)

• Purpose: Amber suppression for incorporating 3-aminotyrosine to proteins in mammalian cells
• Depositing Lab: Huiwang Ai
• Publication: Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038/s41589-020-0641-7. doi: 10.1038/s41589-020-0641-7.

Backbone

• Vector backbone: pMAH
• Backbone size w/o insert (bp): 6960
• Total vector size (bp): 8853
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: EcaYRS
• Alt name: 3-amino-tyrosine synthetase derived from Escherichia coli
• Species: Escherichia coli
• Insert Size (bp): 1893
• GenBank ID:
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: ApaI (unknown if destroyed)
• 5′ sequencing primer: ACA ATT AAT ACA TAA CCT TAT GTA TCA TAC AC
• 3′ sequencing primer: CTG TCC TGC CCC ACC CCA C

Gene/Insert 2

• Gene/Insert name: amber suppression tRNA (multiple copies)
• Species: Synthetic

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry