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(Plasmid #153558)


Item Catalog # Description Quantity Price (USD)
Plasmid 153558 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6960
  • Total vector size (bp) 8853
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Gene/Insert 1

  • Gene/Insert name
  • Alt name
    3-amino-tyrosine synthetase derived from Escherichia coli
  • Species
    Escherichia coli
  • Insert Size (bp)
  • GenBank ID
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site ApaI (unknown if destroyed)
  • 5′ sequencing primer ACA ATT AAT ACA TAA CCT TAT GTA TCA TAC AC
  • 3′ sequencing primer CTG TCC TGC CCC ACC CCA C
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    amber suppression tRNA (multiple copies)
  • Species

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAH-EcaYRS was a gift from Huiwang Ai (Addgene plasmid # 153558 ; ; RRID:Addgene_153558)
  • For your References section:

    A general strategy to red-shift green fluorescent protein-based biosensors. Zhang S, Ai HW. Nat Chem Biol. 2020 Sep 14. pii: 10.1038/s41589-020-0641-7. doi: 10.1038/s41589-020-0641-7. 10.1038/s41589-020-0641-7 PubMed 32929278