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pAH18
(Plasmid #154397)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 154397 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGPI-SceI
  • Backbone manufacturer
    Miguel Valvano
  • Backbone size w/o insert (bp) 4748
  • Total vector size (bp) 5620
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Trimethoprim
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    Trimethoprim 50 ug/mL
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
  • Species
    Synthetic; B. cenocepacia K56-2
  • Insert Size (bp)
    950
  • GenBank ID
    LAUA00000000.1
  • Promoter NA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    NA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the Addgene sequence differs slightly from the author's sequence (.gb file) in non-coding regions of the plasmid. These differences do not alter the plasmid's function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAH18 was a gift from Silvia Cardona (Addgene plasmid # 154397 ; http://n2t.net/addgene:154397 ; RRID:Addgene_154397)
  • For your References section:

    A broad-host-range CRISPRi toolkit for silencing gene expression in Burkholderia. Hogan AM, Rahman ASMZ, Lightly TJ, Cardona ST. ACS Synth Biol. 2019 Sep 6. doi: 10.1021/acssynbio.9b00232. 10.1021/acssynbio.9b00232 PubMed 31491085