Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #129465)


Item Catalog # Description Quantity Price (USD)
Plasmid 129465 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Miguel Valvano and Silvia Cardona
  • Backbone size w/o insert (bp) 5451
  • Total vector size (bp) 5601
  • Modifications to backbone
    pSCB2-sgRNA is the actual vector backbone. Rhamnose-inducible promoter system removed via inverse PCR. Additional inverse PCR to insert random 20 nucleotides as a negative control for no binding of sgRNA to DNA of bacteria.
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    Non-target/random gRNA
  • gRNA/shRNA sequence
  • Species
  • GenBank ID
  • Promoter BBa_J23119 (SpeI)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TAAGATggatccTCAGTTGGCTTCATCGCTAC
  • 3′ sequencing primer CCGCCAGGCAAATTCTGTTT
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    gRNA scaffold was constructed by Qi et al. (2013). Binding sequence (20 nt) was constructed by IDT, ligated in lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Synthetic guide RNA was designed such that it did not bind anywhere on the B. cenocepacia chromosome.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pgRNA-non-target was a gift from Silvia Cardona (Addgene plasmid # 129465 ; ; RRID:Addgene_129465)
  • For your References section:

    A broad-host-range CRISPRi toolkit for silencing gene expression in Burkholderia. Hogan AM, Rahman ASMZ, Lightly TJ, Cardona ST. ACS Synth Biol. 2019 Sep 6. doi: 10.1021/acssynbio.9b00232. 10.1021/acssynbio.9b00232 PubMed 31491085