pYL112-19
(Plasmid
#15459)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15459 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLI50
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Backbone manufacturerlab construct
- Backbone size w/o insert (bp) 5505
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Vector typeS.aureus-E. coli shuttle vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionschloramphenicol/ S. aureus
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameS. aureus phage L54a integrase
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SpeciesS. aureus phage L54a int gene
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Insert Size (bp)1600
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PvuII (destroyed during cloning)
- 3′ cloning site PvuII (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYL112-19 was a gift from Chia Lee (Addgene plasmid # 15459 ; http://n2t.net/addgene:15459 ; RRID:Addgene_15459) -
For your References section:
Improved single-copy integration vectors for Staphylococcus aureus. Luong TT, Lee CY. J Microbiol Methods. 2007 Jul . 70(1):186-90. 10.1016/j.mimet.2007.04.007 PubMed 17512993