str-KDEL_Stx5LdeltaER-SBP-mCitrine
(Plasmid #154846)

• Purpose: Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)
• Depositing Lab: Geert van den Bogaart
• Publication: Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi: 10.1038/s41467-021-26534-y.

Backbone

• Vector backbone: pIRES_neo3
• Backbone manufacturer: Clontech
• Total vector size (bp): 6981
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Stx5 (Long isoform)
• Alt name: STX5
• Alt name: Syntaxin 5
• Alt name: STX5A
• Species: H. sapiens (human)
• Insert Size (bp): 1066
• Mutation: Changed Methionine 55 to Valine, causes loss of Stx5S expression. Changed Arginine 4, Lysine 5 and Arginine 6 to Alanine
• GenBank ID: NM_003164.5
• Entrez Gene: STX5 (a.k.a. CDG2AA, SED5, STX5A)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Streptavidin Binding Protein (SBP) (C terminal on insert)
  • mCitrine (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (not destroyed)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: IRES-F
• 3′ sequencing primer: pEGFP-all

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry