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Congenital disorder of glycosylation caused by starting site-specific variant in syntaxin-5.
Linders PTA, Gerretsen ECF, Ashikov A, Vals MA, de Boer R, Revelo NH, Arts R, Baerenfaenger M, Zijlstra F, Huijben K, Raymond K, Muru K, Fjodorova O, Pajusalu S, Ounap K, Ter Beest M, Lefeber D, van den Bogaart G
Nat Commun. 2021 Oct 28;12(1):6227. doi: 10.1038/s41467-021-26534-y.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
154845str-KDEL_Stx5S-SBP-mCitrineSynchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)
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154846str-KDEL_Stx5LdeltaER-SBP-mCitrineSynchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)
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165082pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1gRNA 1 of pair B for Stx5L-specific knockout
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165083pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2gRNA 2 of pair B for Stx5L-specific knockout
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165085pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1gRNA 1 of pair C for Stx5L-specific knockout
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165086pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2gRNA 2 of pair C for Stx5L-specific knockout
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165087Stx5S-mCherryExpresses Stx5S-mCherry in mammalian cells
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209844Stx5L-mCherryExpresses Stx5L-mCherry in mammalian cells
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209845Stx5L(m55V)-GFPExpresses Stx5 M55V mutant fused to GFP in mammalian cells
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209846str-KDEL_Stx5L(M55V)-SBP-mCitrineSynchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)
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209847Stx5S-GFPExpresses Stx5S-GFP in mammalian cells
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209848Bet1-mCherryExpresses Bet1-mCherry in mammalian cells
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209849Bet1L-mCherryExpresses Bet1L-mCherry in mammalian cells
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209850Bet1-GFPExpresses Bet1-GFP in mammalian cells
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Antibodies from Article