Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15492||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3300
Vector typeXenopus RNA expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameGSK-3 beta
SpeciesR. norvegicus (rat)
Insert Size (bp)1300
/ Fusion Protein
- myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (difficult due to 3' UTR) (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
See author's map for details. 1.4 kb SmaI-HincII rat GSK3beta PCR fragment was cloned from BSSK into SmaI site of pJ3m vector. SalI-BglII digested insert was recloned into XhoI and BglII sites of pXT7; later 3' end was modified to replace SacI, EcoRI, ClaI, and KpnI with XhoI and HindIII.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:rGSKmyc-pXT7 was a gift from Sergei Sokol (Addgene plasmid # 15492 ; http://n2t.net/addgene:15492 ; RRID:Addgene_15492)
For your References section:Role of glycogen synthase kinase 3 beta as a negative regulator of dorsoventral axis formation in Xenopus embryos. Dominguez I, Itoh K, Sokol SY. Proc Natl Acad Sci U S A. 1995 Aug 29. 92(18):8498-502. 10.1073/pnas.92.18.8498 PubMed 7667318
Map uploaded by the depositor.