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(Plasmid #15492)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 15492 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Xenopus RNA expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    GSK-3 beta
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Entrez Gene
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6 (difficult due to 3' UTR)
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for details. 1.4 kb SmaI-HincII rat GSK3beta PCR fragment was cloned from BSSK into SmaI site of pJ3m vector. SalI-BglII digested insert was recloned into XhoI and BglII sites of pXT7; later 3' end was modified to replace SacI, EcoRI, ClaI, and KpnI with XhoI and HindIII.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rGSKmyc-pXT7 was a gift from Sergei Sokol (Addgene plasmid # 15492 ; ; RRID:Addgene_15492)
  • For your References section:

    Role of glycogen synthase kinase 3 beta as a negative regulator of dorsoventral axis formation in Xenopus embryos. Dominguez I, Itoh K, Sokol SY. Proc Natl Acad Sci U S A. 1995 Aug 29. 92(18):8498-502. 10.1073/pnas.92.18.8498 PubMed 7667318