|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||154950||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4817
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)3072
- Promoter human synapsin
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site HindIII (destroyed during cloning)
- 5′ sequencing primer GACTCAGCGCTGCCTCAGTCTG
- 3′ sequencing primer TGTTGCTCCTTTTACGCTATG (Common Sequencing Primers)
The insert contains the fluorescence tag mCerulean3 in between the 2 channelrhodopsins (C-terminal of GtACR2) . Please visit https://www.biorxiv.org/content/10.1101/2020.07.15.204347v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hSyn-DIO-BiPOLES-mCerulean was a gift from Simon Wiegert (Addgene plasmid # 154950 ; http://n2t.net/addgene:154950 ; RRID:Addgene_154950)
For your References section:BiPOLES: a tool for bidirectional dual-color optogenetic control of neurons. Vierock J, Rodriguez-Rozada S, Pieper F, Dieter A, Bergs A, Zeitzschel N, Ahlbeck J, Sauter K, Gottschalk A, Engel AK, Hegemann P, Wiegert JS. bioRxiv (2020) 10.1101/2020.07.15.204347