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(Plasmid #154946)


Item Catalog # Description Quantity Price (USD)
Plasmid 154946 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Promoter minimal Dlx

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site PmeI (destroyed during cloning)
  • 5′ sequencing primer TTGCCTTTCTCTCCACAGGT
  • 3′ sequencing primer TGTTGCTCCTTTTACGCTATG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The insert contains the fluorescence tag mCerulean3 in between the 2 channelrhodopsins (C-terminal of GtACR2) . Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mDlx-BiPOLES-mCerulean was a gift from Simon Wiegert (Addgene plasmid # 154946 ; ; RRID:Addgene_154946)
  • For your References section:

    BiPOLES: a tool for bidirectional dual-color optogenetic control of neurons. Vierock J, Rodriguez-Rozada S, Pieper F, Dieter A, Bergs A, Zeitzschel N, Ahlbeck J, Sauter K, Gottschalk A, Engel AK, Hegemann P, Wiegert JS. bioRxiv (2020) 10.1101/2020.07.15.204347