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HA GST PreScission p70 S6K1
(Plasmid #15511)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15511 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p70 S6K1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1509
  • Entrez Gene
    Rps6kb1 (a.k.a. p70 S6K-alpha)
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA GST PreScission p70 S6K1 was a gift from David Sabatini (Addgene plasmid # 15511 ; http://n2t.net/addgene:15511 ; RRID:Addgene_15511)
  • For your References section:

    PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Sancak Y, Thoreen CC, Peterson TR, Lindquist RA, Kang SA, Spooner E, Carr SA, Sabatini DM. Mol Cell. 2007 Mar 23. 25(6):903-15. 10.1016/j.molcel.2007.03.003 PubMed 17386266