pCMV Aquamarine-shadowY tandem
(Plasmid
#157775)
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Purpose(Empty Backbone) Produces a fusion between Aquamarine and shadowY. It can be used as a positive control for FRET (using FLIM)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 157775 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCMV Aquamarine
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Backbone manufacturerTramier
- Backbone size (bp) 4741
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Vector typeMammalian Expression
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Tags
/ Fusion Proteins
- Aquamarine (N terminal on insert)
- shadowY (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- Aquamarine (N terminal on insert)
- shadowY (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV Aquamarine-shadowY tandem was a gift from Marc Tramier (Addgene plasmid # 157775 ; http://n2t.net/addgene:157775 ; RRID:Addgene_157775) -
For your References section:
Aurora kinase A localises to mitochondria to control organelle dynamics and energy production. Bertolin G, Bulteau AL, Alves-Guerra MC, Burel A, Lavault MT, Gavard O, Le Bras S, Gagne JP, Poirier GG, Le Borgne R, Prigent C, Tramier M. eLife. 2018 Aug 2;7. pii: 38111. doi: 10.7554/eLife.38111. 10.7554/eLife.38111 PubMed 30070631
