pJ609-dCas9-KR-puro
(Plasmid
#158478)
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PurposeExpress nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158478 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepJ609
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Backbone manufacturerDNA2.0
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Vector typeMammalian Expression, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9-KR
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SpeciesSynthetic
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Insert Size (bp)4899
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MutationCas9m4 (D10A+D839A+H840A+N863A)
- Promoter CMV
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Tag
/ Fusion Protein
- KillerRed (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDNA2.0
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cas9m4 sequence taken from Addgene plasmid #47316; KillerRed sequence from Evrogen (http://evrogen.com/products/KillerRed/KillerRed_Detailed_description.shtml). KillerRed is fused to Cas9m4 with a 5 amino acid linker.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJ609-dCas9-KR-puro was a gift from Brendan Price (Addgene plasmid # 158478 ; http://n2t.net/addgene:158478 ; RRID:Addgene_158478)