p5E-1.7kbfabp6
(Plasmid
#159087)
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Purposep5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 159087 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonep5E-Fse-Asc entry vector
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Backbone manufacturertol2kit
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Vector typepromoter fragment 5' entry vector for tol2 cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name1.7kb fabp6 promoter
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1700
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Entrez Genefabp6 (a.k.a. zgc:92421)
- Promoter 1.7kb fabp6
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer ttaaggccggccGATGATCCCAACCCTGTAATGAGTTCCTGG
- 3′ sequencing primer aattggcgcgccTTGAGAGCTGAGGTACTGATGGGTGAAG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5E-1.7kbfabp6 was a gift from John Rawls (Addgene plasmid # 159087 ; http://n2t.net/addgene:159087 ; RRID:Addgene_159087) -
For your References section:
Fxr signaling and microbial metabolism of bile salts in the zebrafish intestine. Wen J, Mercado GP, Volland A, Doden HL, Lickwar CR, Crooks T, Kakiyama G, Kelly C, Cocchiaro JL, Ridlon JM, Rawls JF. Sci Adv. 2021 Jul 23;7(30). pii: 7/30/eabg1371. doi: 10.1126/sciadv.abg1371. Print 2021 Jul. 10.1126/sciadv.abg1371 PubMed 34301599
