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Zhou-COVID-19-Spike
(Plasmid #161029)

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Item Catalog # Description Quantity Price (USD)
Plasmid 161029 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 161029-D50 50 μg of DNA in Tris buffer $495 *
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DNA 161029-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    PCMV3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Engineered COVID-19 Spike protein, VSV-G
  • Species
    SAR-CoV-2, Vesicular stomatitis virus
  • Mutation
    Codon optimized
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also contains signal peptide of VSV G protein and the transmembrane and intracellular regions of VSV G protein.

Please visit https://www.biorxiv.org/content/10.1101/2021.02.17.431647v1 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Zhou-COVID-19-Spike was a gift from Dapeng Zhou (Addgene plasmid # 161029 ; http://n2t.net/addgene:161029 ; RRID:Addgene_161029)

  • For your References section:

    Potent Neutralization Antibodies Induced by a Recombinant Trimeric Spike Protein Vaccine Candidate Containing PIKA Adjuvant for COVID-19. Tong J, Zhu C, Lai H, Feng C, Zhou D. Vaccines (Basel). 2021 Mar 22;9(3). pii: vaccines9030296. doi: 10.3390/vaccines9030296. 10.3390/vaccines9030296 PubMed 33810026
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