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PurposeExpresses GFP-tagged PH-FAPP1. PI(4)P biosensor
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161986 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneeGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePH-FAPP1
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Alt namePLEKHA3
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SpeciesH. sapiens (human)
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Entrez GenePLEKHA3 (a.k.a. FAPP1)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-PH-FAPP1 was a gift from Tamas Balla & David Bryant (Addgene plasmid # 161986 ; http://n2t.net/addgene:161986 ; RRID:Addgene_161986) -
For your References section:
A plasma membrane pool of phosphatidylinositol 4-phosphate is generated by phosphatidylinositol 4-kinase type-III alpha: studies with the PH domains of the oxysterol binding protein and FAPP1. Balla A, Tuymetova G, Tsiomenko A, Varnai P, Balla T. Mol Biol Cell. 2005 Mar;16(3):1282-95. doi: 10.1091/mbc.e04-07-0578. Epub 2005 Jan 5. 10.1091/mbc.e04-07-0578 PubMed 15635101
