FastLightR-bRaf-mVenus
(Plasmid
#162155)
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PurposeExpresses FastLightR-bRaf-mVenus in mammalian cells
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Depositing Lab
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Depositing OrganizationUniversity of Illinois at Chicago
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162155 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 162155-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 162155-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneNot sure
- Backbone size w/o insert (bp) 3934
- Total vector size (bp) 8008
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFastLightR-bRaf-mVenus
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SpeciesM. musculus (mouse)
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Insert Size (bp)4074
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MutationValine 600 is substituted with Glutamic acid
- Promoter CMV
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Tag
/ Fusion Protein
- mVenus (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-Fw
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 162155-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 162155-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FastLightR-bRaf-mVenus was a gift from Andrei Karginov (Addgene plasmid # 162155 ; http://n2t.net/addgene:162155 ; RRID:Addgene_162155) -
For your References section:
Light-Regulated allosteric switch enables temporal and subcellular control of enzyme activity. Shaaya M, Fauser J, Zhurikhina A, Conage-Pough JE, Huyot V, Brennan M, Flower CT, Matsche J, Khan S, Natarajan V, Rehman J, Kota P, White FM, Tsygankov D, Karginov AV. Elife. 2020 Sep 23;9. pii: 60647. doi: 10.7554/eLife.60647. 10.7554/eLife.60647 PubMed 32965214
Map uploaded by the depositor.
