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pBa.NgCAM-Halo
(Plasmid #162719)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162719 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 162719-D50 50 μg of DNA in Tris buffer $495
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DNA 162719-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBeta Actin-Halo
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NgCam
  • Species
    G. gallus (chicken)
  • Mutation
    E137K, K138E, and K642E
  • GenBank ID
    Z75013
  • Entrez Gene
    L1CAM
  • Promoter Chicken Beta actin
  • Tag / Fusion Protein
    • Halo (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBa.NgCAM-Halo was a gift from Marvin Bentley (Addgene plasmid # 162719 ; http://n2t.net/addgene:162719 ; RRID:Addgene_162719)

  • For your References section:

    A Novel Labeling Strategy Reveals That Myosin Va and Myosin Vb Bind the Same Dendritically Polarized Vesicle Population. Frank M, Citarella CG, Quinones GB, Bentley M. Traffic. 2020 Sep 21. doi: 10.1111/tra.12764. 10.1111/tra.12764 PubMed 32959500
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