PurposeSeparately expressing 3 unique sgIGR_dual-MTS (SetI)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||162764||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert namesgIGR_dual-MTS_1; sgIGR_dual-MTS_2; sgIGR_dual-MTS_3
SpeciesH. sapiens (human)
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEM-sgIGR_dual-MTS-3x-Set I was a gift from Antony K Chen (Addgene plasmid # 162764 ; http://n2t.net/addgene:162764 ; RRID:Addgene_162764)
For your References section:CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci. Mao S, Ying Y, Wu X, Krueger CJ, Chen AK. Nucleic Acids Res. 2019 Nov 18;47(20):e131. doi: 10.1093/nar/gkz752. 10.1093/nar/gkz752 PubMed 31504824