p1.2-GS-eGFP
(Plasmid
#162771)
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PurposeFluorescent reporter for CHO expression studies
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Depositing Lab
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Depositing OrganizationFederal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences
Located in the Russian Federation -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162771 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 162771-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 162771-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonep1.1
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Backbone manufacturerLMBT, LTD
- Backbone size w/o insert (bp) 11914
- Total vector size (bp) 13510
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Modifications to backboneThe region containing the EMCV IRES and the DHFR ORF from the p1.1 expression vector is removed. The GS ORF and the SV40 promoter and terminator regions are added.
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Vector typeMammalian Expression
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Selectable markersL-Methionine sulfoximine (MSX)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameenhanced green fluorescent protein
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Alt nameeGFP
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SpeciesAequorea victoria
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Insert Size (bp)735
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Mutationconsensus Kozak sequence (GCCGCCATGG) added before the ORF
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GenBank IDAAB02576.1
- Promoter CHO EEF1A1 (Translation Elongation Factor 1 Alpha 1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AbsI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer SQ-5CH6-F GCCGCTGCTTCCTGTGAC
- 3′ sequencing primer SQ-3CH1-R ACAAACAGTTCTGAGACCG
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byInsert made by PCR with pEGFPN2 (Clontech) as a template
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 162771-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 162771-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p1.2-GS-eGFP was a gift from Ivan Vorobiev (Addgene plasmid # 162771 ; http://n2t.net/addgene:162771 ; RRID:Addgene_162771) -
For your References section:
Approaches to Controlled Co-Amplification of Genes for Production of Biopharmaceuticals: Study of the Insertion and Amplification Dynamics of Genetic Cassettes in the Genome of Chinese Hamster Ovary Cells during Co-Expression of Compatible Pair of Plasmids. Kovnir SV, Orlova NA, Khodak Ycapital A, Cyrillic, Kondrashova MP, Gabibov AG, Skryabin KG, Vorobiev AI, Vorobiev II. Bull Exp Biol Med. 2017 Jun;163(2):245-249. doi: 10.1007/s10517-017-3776-0. Epub 2017 Jul 18. 10.1007/s10517-017-3776-0 PubMed 28726207
Map uploaded by the depositor.
