pSCrhaB2plus
(Plasmid #164226)

• Purpose: (Empty Backbone) Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.
• Depositing Lab: Silvia Cardona
• Publication: Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0064721. doi: 10.1128/AEM.00647-21.

Backbone

• Vector backbone: pSCrhaB2
• Backbone manufacturer: Miguel Valvano and Silvia Cardona (Addgene plasmid # 113634)
• Backbone size (bp): 7518
• Modifications to backbone: Upstream of PrhaBAD, exchanged rhaI2 site for rhaI1 (resulting in two rhaI1 sites). Downstream of PrhaBAD, added T7 gene 10 stem loop as 5' extension of mRNA.
• Vector type: Bacterial Expression
• Promoter: Rhamnose-inducible

Growth in Bacteria

• Bacterial Resistance(s): Trimethoprim
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• 3′ sequencing primer: pBAD reverse

Resource Information

• Supplemental Documents:
  • pSCrhaB2plus.gb
• A portion of this plasmid was derived from a plasmid made by: Miguel Valvano and Silvia Cardona at Western University. This plasmid is derived from pSCrhaB2 (Addgene plasmid #113634).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is derived from pSCrhaB2 (Addgene plasmid #113634).
Reference for pSCrhaB2:
Cardona ST, Valvano MA. Plasmid. 2005 Nov;54(3):219-28. doi: 10.1016/j.plasmid.2005.03.004