pαH-S-HRV3C/D614G
(Plasmid
#164568)
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PurposeSARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)
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Depositing Lab
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Depositing OrganizationDuke University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164568 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 164568-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 164568-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepαH
- Backbone size w/o insert (bp) 4722
- Total vector size (bp) 8358
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpike (S-HRV3C-D614G variant)
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SpeciesSevere acute respiratory syndrome coronavirus 2
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Insert Size (bp)3636
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MutationD614G
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GenBank IDMN908947
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Tags
/ Fusion Proteins
- 8X His tag (C terminal on backbone)
- 2X Strep-Tag II (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 164568-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 164568-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pαH-S-HRV3C/D614G was a gift from Priyamvada Acharya (Addgene plasmid # 164568 ; http://n2t.net/addgene:164568 ; RRID:Addgene_164568) -
For your References section:
D614G Mutation Alters SARS-CoV-2 Spike Conformation and Enhances Protease Cleavage at the S1/S2 Junction. Gobeil SM, Janowska K, McDowell S, Mansouri K, Parks R, Manne K, Stalls V, Kopp MF, Henderson R, Edwards RJ, Haynes BF, Acharya P. Cell Rep. 2021 Jan 12;34(2):108630. doi: 10.1016/j.celrep.2020.108630. Epub 2020 Dec 26. 10.1016/j.celrep.2020.108630 PubMed 33417835
Map uploaded by the depositor.
