pTA231-p.Syn-PAmCherry1Hfx
(Plasmid
#164661)
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Purposeexpression of H. volcanii codon-optimized PAmCherry1
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Depositing Lab
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Depositing OrganizationUniversitaet Ulm
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 164661 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 164661-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 164661-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTA231-p.Syn
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Backbone manufacturerBerkemer et al., 2020, Identification of RNA 3´ ends and termination sites in Haloferax volcanii
- Backbone size w/o insert (bp) 7765
- Total vector size (bp) 8476
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Vector typearchaeal expression
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Selectable markersmarker for H. volcanii: trpA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePAmCherry1Hfx
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Alt nameH. volcanii codon-optimized PAmCherry1
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SpeciesSynthetic
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Insert Size (bp)711
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Mutationcodon-optimization for Haloferax volcanii
- Promoter p.Syn
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer M13
- 3′ sequencing primer M13rev
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPAmCherry1Hfx ordered from GeneArt for synthesis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 164661-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 164661-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTA231-p.Syn-PAmCherry1Hfx was a gift from Anita Marchfelder (Addgene plasmid # 164661 ; http://n2t.net/addgene:164661 ; RRID:Addgene_164661) -
For your References section:
Establishing Live-Cell Single-Molecule Localization Microscopy Imaging and Single-Particle Tracking in the Archaeon Haloferax volcanii. Turkowyd B, Schreiber S, Wortz J, Segal ES, Mevarech M, Duggin IG, Marchfelder A, Endesfelder U. Front Microbiol. 2020 Nov 20;11:583010. doi: 10.3389/fmicb.2020.583010. eCollection 2020. 10.3389/fmicb.2020.583010 PubMed 33329447
Map uploaded by the depositor.
