PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||164965||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4179
- Total vector size (bp) 12696
Vector typeTol2 transposon destination vector
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)1479
Entrez Genekcna1a (a.k.a. Kv1., kcn, kcna1, si:ch73-333m22.1, wu:fa27a, wu:fa27a01)
- Promoter actinb (actb2) promoter
/ Fusion Protein
- IRES-EGFP (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13R
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The DNA sequence was amplified from pooled wild type TAB zebrafish embryos. There is a likely polymorphism, K278M, which is not listed in the current ENSEMBL database. This is possibly due to incomplete zebrafish genome annotation, GRCz11.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDestTol2-actinb-kcna1a-IRES-EGFP was a gift from GuangJun Zhang (Addgene plasmid # 164965 ; http://n2t.net/addgene:164965 ; RRID:Addgene_164965)
For your References section:Potassium Channel-Associated Bioelectricity of the Dermomyotome Determines Fin Patterning in Zebrafish. Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, Mittal SK, Zhang G. Genetics. 2020 Aug;215(4):1067-1084. doi: 10.1534/genetics.120.303390. Epub 2020 Jun 16. 10.1534/genetics.120.303390 PubMed 32546498