Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16514||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9200
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2400
Entrez GeneTCF7L2 (a.k.a. TCF-4, TCF4)
/ Fusion Protein
- Myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.
According to the depositing scientist, other mutations may be present but they have no functional consequences on standard assays for Tcf4 activity.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHR TCF4 was a gift from Bert Vogelstein (Addgene plasmid # 16514 ; http://n2t.net/addgene:16514 ; RRID:Addgene_16514)
For your References section:Constitutive transcriptional activation by a beta-catenin-Tcf complex in APC-/- colon carcinoma. Korinek V, Barker N, Morin PJ, van Wichen D, de Weger R, Kinzler KW, Vogelstein B, Clevers H. Science. 1997 Mar 21. 275(5307):1784-7. 10.1126/science.275.5307.1784 PubMed 9065401
Map uploaded by the depositor.