Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
We have implemented updates to our Transparency and Privacy Policy.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

tTA-IRES-Neo
(Plasmid #16541)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16541 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMk10-59
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tTA
  • Alt name
    tetracycline-controlled transactivator
  • Insert Size (bp)
    1000

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

tTA is a a tetracycline-controlled transactivator generated by fusing the tet repressor with the activating domain of virion protein 16 of herpes simplex virus. tTA-IRES-Neo was generated by cloning PCR-amplified tTA cDNA from pUHD15-1 (Gossen, M. & Bujard, H., 1992, PNAS 89, 5547-5551) into the XbaI site of plasmid pMk10-59 (Kobayashi, M et al., 1996, Biotechniques 21, 398-402).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tTA-IRES-Neo was a gift from Bert Vogelstein (Addgene plasmid # 16541 ; http://n2t.net/addgene:16541 ; RRID:Addgene_16541)
  • For your References section:

    Identification and classification of p53-regulated genes. Yu J, Zhang L, Hwang PM, Rago C, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1999 Dec 7. 96(25):14517-22. 10.1073/pnas.96.25.14517 PubMed 10588737