Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16554||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 7462
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneCDX2 (a.k.a. CDX-3, CDX2/AS, CDX3)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
RKO (colorectal cancer cell line) mutation: 4 base-pair deletion (AGGCAGG to AGG) within the sequence corresponding to helix 3 of the CDX2 homeodomain.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CMV-CDX2-MUT was a gift from Bert Vogelstein (Addgene plasmid # 16554 ; http://n2t.net/addgene:16554 ; RRID:Addgene_16554)
For your References section:CDX2 is mutated in a colorectal cancer with normal APC/beta-catenin signaling. da Costa LT, He TC, Yu J, Sparks AB, Morin PJ, Polyak K, Laken S, Vogelstein B, Kinzler KW. Oncogene. 1999 Sep 2. 18(35):5010-4. 10.1038/sj.onc.1202872 PubMed 10490837
Map uploaded by the depositor.