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(Plasmid #165612)


Item Catalog # Description Quantity Price (USD)
Plasmid 165612 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Church Lab (Addgene #43803)
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy


  • Gene/Insert name
    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
  • Mutation
    NotI site replaced the CAN1 spacer in parent vector (to facilitate insertion of new spacers via Gibson assembly)
  • Promoter SNR52

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was constructed by Dr. Agmon in the Boeke lab and first described in:
Agmon, N. et al. Phylogenetic debugging of a complete human biosynthetic pathway transplanted into yeast. Nucleic Acids Res. 48, 486–499 (2020)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNA0304 was a gift from Marcus Noyes (Addgene plasmid # 165612 ; ; RRID:Addgene_165612)
  • For your References section:

    Engineered dual selection for directed evolution of SpCas9 PAM specificity. Goldberg GW, Spencer JM, Giganti DO, Camellato BR, Agmon N, Ichikawa DM, Boeke JD, Noyes MB. Nat Commun. 2021 Jan 13;12(1):349. doi: 10.1038/s41467-020-20650-x. 10.1038/s41467-020-20650-x PubMed 33441553