pBS L30 Flag-Apex2-Rab18
(Plasmid
#166403)
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PurposeRab18 fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166403 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBS
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Modifications to backboneModified for mammalian expression using the promoter of the mouse ribosomal protein L30 gene ("weak promoter") and a HSV TK polyA tail
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRab18
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Alt nameRab18
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SpeciesH. sapiens (human)
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GenBank ID22931
- Promoter L30 weak promoter
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Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- APEX2 (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer T3
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.10.07.680694 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS L30 Flag-Apex2-Rab18 was a gift from Raphael Gaudin (Addgene plasmid # 166403 ; http://n2t.net/addgene:166403 ; RRID:Addgene_166403) -
For your References section:
Monitoring the dynamics of exosomes through the tracking of Rab7 partners. Bare Y, Delalande F, Hirschler A, Cazevieille C, Lucansky V, Carapito C, Gaudin R, Deffieu MS. bioRxiv 2025.10.07.680694 10.1101/2025.10.07.680694