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(Plasmid #166671)


Item Catalog # Description Quantity Price (USD)
Plasmid 166671 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Virginia Lee
  • Modifications to backbone
    A TEV site (ENLYFQG) was inserted following the 6x His tag, in frame with the start of the GFP sequence.
  • Vector type
    Bacterial Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    While DH5alpha can be used for plasmid maintenance and storage, BL21(DE3) should be used for protein production. We used an autoinduction protocol.
  • Copy number
    Low Copy


  • Gene/Insert name
    mouse synuclein (from V. Lee)
  • Alt name
  • Species
    M. musculus (mouse)
  • Mutation
    added a TEV cleavage site sequence between synuclein and GFP
  • Entrez Gene
    Snca (a.k.a. NACP, alpha-Syn, alphaSYN)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Virginia Lee, University of Pennsylvania
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The original plasmid reference is:
"Selective imaging of internalized proteopathic a-synuclein seeds in primary neurons reveals mechanistic insight into transmission of synucleinopathies" JBC 2017, DOI 0.1074/jbc.M117.780296
Richard J. Karpowicz, Jr. , Conor M. Haney , Tiberiu S. Mihaila , Raizel M. Sandler , E. James Petersson, and Virginia M.-Y. Lee

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK172-α-syn-TEV-GFP was a gift from Iris Lindberg (Addgene plasmid # 166671 ; ; RRID:Addgene_166671)
  • For your References section:

    A protease protection assay for the detection of internalized alpha-synuclein pre-formed fibrils. Jarvela TS, Chaplot K, Lindberg I. PLoS One. 2021 Jan 26;16(1):e0241161. doi: 10.1371/journal.pone.0241161. eCollection 2021. 10.1371/journal.pone.0241161 PubMed 33497415