1a. pETM11-SUMO-SNCA-GFP
(Plasmid #107292)

• Purpose: Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
• Depositing Lab: Dmytro Yushchenko
• Publication: Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191. doi: 10.1016/j.bpj.2017.08.027. Epub 2017 Sep 20.

Backbone

• Vector backbone: pETM11
• Vector type: Bacterial Expression
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SNCA-GFP
• Species: H. sapiens (human)
• Insert Size (bp): 1170
• Entrez Gene: SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
• Promoter: T7
• Tag / Fusion Protein:
  • His-tag and SUMO-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: T-7 primer
• 3′ sequencing primer: TATATACTGCTTGTTGCTGATGGCGTC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry