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pLSB-HYG
(Plasmid #166699)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 166699 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLSB
  • Backbone size w/o insert (bp) 5444
  • Total vector size (bp) 12001
  • Vector type
    Yeast Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    tRNA promoter : sgRNA cassette
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    1393
  • Promoter tRNA promoter

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    adh15 promoter : Cas9 codon-optimised for S. pombe
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    5164
  • Promoter adh15 promoter

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The following acknowledgement must be included in all research publications and other outputs: “pLSB-HYG was created with the support of the Wellcome Trust [095021], [200885], [203149]”.

The following statement must be included on all submissions of original research to peer-reviewed journals: “pLSB-HYG was created with the support of the Wellcome Trust [095021], [200885], [203149]”.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLSB-HYG was a gift from Robin Allshire (Addgene plasmid # 166699 ; http://n2t.net/addgene:166699 ; RRID:Addgene_166699)
  • For your References section:

    SpEDIT: A fast and efficient CRISPR/Cas9 method for fission yeast. Torres-Garcia S, Di Pompeo L, Eivers L, Gaborieau B, White SA, Pidoux AL, Kanigowska P, Yaseen I, Cai Y, Allshire RC. Wellcome Open Res. 2020 Nov 24;5:274. doi: 10.12688/wellcomeopenres.16405.1. eCollection 2020. 10.12688/wellcomeopenres.16405.1 PubMed 33313420