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pTJV1Sc-rpoB
(Plasmid #166978)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 166978 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pKDsgRNA
  • Backbone manufacturer
    Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 5900
  • Modifications to backbone
    removed lambda exo, bet, and gam
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Ec86 Retron
  • Insert Size (bp)
    1200
  • GenBank ID
    KM923747.1
  • Entrez Gene
    ECD_00831 (a.k.a. ECD_00831)
  • Promoter J23101

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tccccgaaaagtgccacc
  • 3′ sequencing primer CAACCGGATCCTTAGGTCT
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    lambda bet
  • Insert Size (bp)
    800
  • GenBank ID
    KM923747.1
  • Promoter VanCC

Cloning Information for Gene/Insert 2

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTJV1Sc-rpoB was a gift from Christopher Reisch (Addgene plasmid # 166978 ; http://n2t.net/addgene:166978 ; RRID:Addgene_166978)
  • For your References section:

    Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli. Ellington AJ, Reisch CR. Synth Biol (Oxf). 2022 May 3;7(1):ysac007. doi: 10.1093/synbio/ysac007. eCollection 2022. 10.1093/synbio/ysac007 PubMed 35673614