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Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli.
Ellington AJ, Reisch CR
Synth Biol (Oxf). 2022 May 3;7(1):ysac007. doi: 10.1093/synbio/ysac007. eCollection 2022. PubMed Article

Plasmids from Article

ID Plasmid Purpose
166978pTJV1Sc-rpoBGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
166979pTJV1ScGm-rpoBGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aacC1 for gentamycin resistance
166980pTJV1ScKm-rpoBGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance
166981pCas9CyMutLEncodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair
166982pTJV1Sc2-tetAGenerates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
166983pTJV1Sc3-rpoBGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

Antibodies from Article