CRISPRoff-v2.1 Citations (19)
Originally described in: Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing.Nuñez JK, Chen J, Pommier GC, Cogan JZ, Replogle JM, Adriaens C, Ramadoss GN, Shi Q, Hung KL, Samelson AJ, Pogson AN, Kim JYS, Chung A, Leonetti MD, Chang HY, Kampmann M, Bernstein BE, Hovestadt V, Gilbert LA, Weissman JS Cell. 2021 Apr 7. pii: S0092-8674(21)00353-6. doi: 10.1016/j.cell.2021.03.025. PubMed Journal
Articles Citing CRISPRoff-v2.1
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| Determinants of heritable gene silencing for KRAB-dCas9 + DNMT3 and Ezh2-dCas9 + DNMT3 hit-and-run epigenome editing. O'Geen H, Tomkova M, Combs JA, Tilley EK, Segal DJ. Nucleic Acids Res. 2022 Apr 8;50(6):3239-3253. doi: 10.1093/nar/gkac123. PubMed |
| Nuclear receptor ligand screening in an iPSC-derived in vitro blood-brain barrier model identifies new contributors to leptin transport. Shi Y, Kim H, Hamann CA, Rhea EM, Brunger JM, Lippmann ES. Fluids Barriers CNS. 2022 Sep 21;19(1):77. doi: 10.1186/s12987-022-00375-3. PubMed |
| Functional Validation of the Putative Oncogenic Activity of PLAU. Sarno F, Goubert D, Logie E, Rutten MGS, Koncz M, Deben C, Niemarkt AE, Altucci L, Verschure PJ, Kiss A, Berghe WV, Rots MG. Biomedicines. 2022 Dec 30;11(1):102. doi: 10.3390/biomedicines11010102. PubMed |
| Downregulation of MGMT expression by targeted editing of DNA methylation enhances temozolomide sensitivity in glioblastoma. Han X, Abdallah MOE, Breuer P, Stahl F, Bakhit Y, Potthoff AL, Pregler BEF, Schneider M, Waha A, Wullner U, Evert BO. Neoplasia. 2023 Oct;44:100929. doi: 10.1016/j.neo.2023.100929. Epub 2023 Aug 25. PubMed |
| Epigenetic modification and characterization of the MGMT promoter region using CRISPRoff in glioblastoma cells. Weber R, Weller M, Reifenberger G, Vasella F. Front Oncol. 2024 Jan 31;14:1342114. doi: 10.3389/fonc.2024.1342114. eCollection 2024. PubMed |
| Chromatin context-dependent regulation and epigenetic manipulation of prime editing. Li X, Chen W, Martin BK, Calderon D, Lee C, Choi J, Chardon FM, McDiarmid TA, Daza RM, Kim H, Lalanne JB, Nathans JF, Lee DS, Shendure J. Cell. 2024 May 9;187(10):2411-2427.e25. doi: 10.1016/j.cell.2024.03.020. Epub 2024 Apr 11. PubMed |
| Epigenetic editing of BRCA1 promoter increases cisplatin and olaparib sensitivity of ovarian cancer cells. He W, Zhu H, Zhang S, Shu G, Lei H, Wang M, Yin G, Ni X, Wu Q. Epigenetics. 2024 Dec;19(1):2357518. doi: 10.1080/15592294.2024.2357518. Epub 2024 May 26. PubMed |
| Epigenome editing revealed the role of DNA methylation of T-DMR/CpG island shore on Runx2 transcription. Kawa Y, Shindo M, Ohgane J, Inui M. Biochem Biophys Rep. 2024 May 17;38:101733. doi: 10.1016/j.bbrep.2024.101733. eCollection 2024 Jul. PubMed |
| Alzheimer's disease risk allele of PICALM causes detrimental lipid droplets in microglia. Kozlova A, Zhang S, Sudwarts A, Zhang H, Smirnou S, Sun X, Stephenson K, Zhao X, Jamison B, Ponnusamy M, He X, Pang ZP, Sanders AR, Bellen HJ, Thinakaran G, Duan J. Res Sq [Preprint]. 2024 May 24:rs.3.rs-4407146. doi: 10.21203/rs.3.rs-4407146/v1. PubMed |
| CRISPRoff epigenome editing for programmable gene silencing in human cell lines and primary T cells. Pattali RK, Ornelas IJ, Nguyen CD, Xu D, Divekar NS, Nunez NK. Methods Enzymol. 2025;712:517-551. doi: 10.1016/bs.mie.2025.01.010. Epub 2025 Mar 6. PubMed |
| Epigenetic editing at individual age-associated CpGs affects the genome-wide epigenetic aging landscape. Liesenfelder S, Elsafi Mabrouk MH, Iliescu J, Baranda MV, Mizi A, Perez-Correa JF, Wessiepe M, Papantonis A, Wagner W. Nat Aging. 2025 Jun;5(6):997-1009. doi: 10.1038/s43587-025-00841-1. Epub 2025 Mar 24. PubMed |
| dFLASH; dual FLuorescent transcription factor activity sensor for histone integrated live-cell reporting and high-content screening. Allen TP, Roennfeldt AE, Reckdharajkumar M, Sullivan AE, Liu M, Quinn RJ, Russell DL, Peet DJ, Whitelaw ML, Bersten DC. Nat Commun. 2025 Apr 7;16(1):3298. doi: 10.1038/s41467-025-58488-w. PubMed |
| The ZBTB24-CDCA7-HELLS axis suppresses the totipotent 2C-like reprogramming by maintaining Dux methylation and repression. Guo D, Du Z, Liu Y, Lin M, Lu Y, Hardikar S, Xue Y, Zhang J, Chen T, Dan J. Nucleic Acids Res. 2025 Apr 10;53(7):gkaf302. doi: 10.1093/nar/gkaf302. PubMed |
| Elucidating the genetic mechanisms governing cytosine base editing outcomes through CRISPRi screens. Gu S, Bodai Z, Anderson RA, So HYA, Cowan QT, Komor AC. Nat Commun. 2025 May 20;16(1):4685. doi: 10.1038/s41467-025-59948-z. PubMed |
| Durable HTT silencing using non-evolved dCas9 epigenome editors in patient-derived cells. Waldo JJ, Halmai JANM, Singh A, Gonzalez CE, Chen YA, Carthen SA, Nolta JA, Fink KD. Mol Ther Nucleic Acids. 2025 May 14;36(2):102561. doi: 10.1016/j.omtn.2025.102561. eCollection 2025 Jun 10. PubMed |
| Epigenetic editing and epi-drugs: a combination strategy to simultaneously target KDM4 as a novel anticancer approach. Sarno F, Jacob JJ, Eilers RE, Nebbioso A, Altucci L, Rots MG. Clin Epigenetics. 2025 Jun 19;17(1):105. doi: 10.1186/s13148-025-01913-0. PubMed |
| Removal of promoter CpG methylation by epigenome editing reverses HBG silencing. Bell HW, Feng R, Shah M, Yao Y, Douglas J, Doerfler PA, Mayuranathan T, O'Dea MF, Li Y, Wang YD, Zhang J, Mackay JP, Cheng Y, Quinlan KGR, Weiss MJ, Crossley M. Nat Commun. 2025 Jul 27;16(1):6919. doi: 10.1038/s41467-025-62177-z. PubMed |
Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins.
Xu D, Besselink S, Ramadoss GN, Dierks PH, Lubin JP, Pattali RK, Brim JI, Christenson AE, Colias PJ, Ornelas IJ, Nguyen CD, Chasins SE, Conklin BR, Nunez JK.
Nat Commun. 2025 Aug 26;16(1):7948. doi: 10.1038/s41467-025-63167-x.
PubMed
Associated Plasmids |
| PICALM Alzheimer's risk allele causes aberrant lipid droplets in microglia. Kozlova A, Zhang S, Sudwarts A, Zhang H, Smirnou S, Byeon SK, Thapa C, Sun X, Stephenson K, Zhao X, Jamison B, Ponnusamy M, He X, Schneider JA, Pandey A, Bennett DA, Pang ZP, Sanders AR, Bellen HJ, Thinakaran G, Duan J. Nature. 2025 Oct;646(8087):1178-1186. doi: 10.1038/s41586-025-09486-x. Epub 2025 Sep 3. PubMed |
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