|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16815||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeXenopus Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
MutationF36M in FKBP
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This is a fusion protein consisting of a FKBP domain at the amino terminus of rat Frizzled 1, and GFP at the C terminus.
The expression of the protein is under the control of a CMV promoter. Once expressed the fusion protein enters the endoplasmic reticulum, where it remains trapped (and acts as a dominant negative Frizzled by trapping endogenous Frizzleds) unless one adds proprietary Ariad small molecule reagents that bind to and unfold the FKBP domain, allowing proteolysis and then processing and membrane targeting of the Frizzled 1. Note that the Frizzleds interact in a homo and heteromeric manner.
Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE240 Fz1-ER-GFP was a gift from Randall Moon (Addgene plasmid # 16815 ; http://n2t.net/addgene:16815 ; RRID:Addgene_16815)