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XE240 Fz1-ER-GFP
(Plasmid #16815)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16815 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fz1-ER-GFP
  • Alt name
    Fz1
  • Species
    R. norvegicus (rat)
  • Mutation
    F36M in FKBP
  • Tag / Fusion Protein
    • gfp

Cloning Information

Resource Information

Depositor Comments

This is a fusion protein consisting of a FKBP domain at the amino terminus of rat Frizzled 1, and GFP at the C terminus.
The expression of the protein is under the control of a CMV promoter. Once expressed the fusion protein enters the endoplasmic reticulum, where it remains trapped (and acts as a dominant negative Frizzled by trapping endogenous Frizzleds) unless one adds proprietary Ariad small molecule reagents that bind to and unfold the FKBP domain, allowing proteolysis and then processing and membrane targeting of the Frizzled 1. Note that the Frizzleds interact in a homo and heteromeric manner.

Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE240 Fz1-ER-GFP was a gift from Randall Moon (Addgene plasmid # 16815 ; http://n2t.net/addgene:16815 ; RRID:Addgene_16815)