PurposeExpression of human plus-TIP microtubule binding protein EB3 fused with mEmerald for microtubule +end live tracking
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||168514||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneMAPRE3 (a.k.a. EB3, EBF3, EBF3-S, RP3)
- Promoter CAG
/ Fusion Protein
- mEmerald (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG mEmerald-EB3 was a gift from Franck Polleux (Addgene plasmid # 168514 ; http://n2t.net/addgene:168514 ; RRID:Addgene_168514)
For your References section:Progressive Decrease of Mitochondrial Motility during Maturation of Cortical Axons In Vitro and In Vivo. Lewis TL Jr, Turi GF, Kwon SK, Losonczy A, Polleux F. Curr Biol. 2016 Oct 10;26(19):2602-2608. doi: 10.1016/j.cub.2016.07.064. Epub 2016 Sep 15. 10.1016/j.cub.2016.07.064 PubMed 27641765