pSP64T-dnWnt-11
(Plasmid #16857)

• Depositing Lab: Jim Smith
• Depositing Organization: MRC National Institute for Medical Research (Inactive)
  Located in United Kingdom of Great Britain and Northern Ireland
• Publication: Tada et al Development. 2000 May . 127(10):2227-38.

Backbone

• Vector backbone: pSP64T
• Backbone manufacturer: Available at Addgene (#15030)
• Backbone size w/o insert (bp): 3300
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dnWnt-11
• Alt name: Wnt11
• Alt name: Xwnt11
• Species: X. laevis (frog)
• Insert Size (bp): 850
• Mutation: amino acids 1-282. Dominant Negative.
• Entrez Gene: wnt11b.L (a.k.a. XELAEV_18038627mg, WNT11, wnt-11, wnt11b, xwnt11)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: BglII (unknown if destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A C-terminally truncated dominant-negative Xwnt11 construct (comprising amino acids 1-282) was generated according to Hoppler
et al. (1996). PCR was carried out using pGEM-X9 (Ku and Melton, 1993) as a template and the primers 5′-CCCCCTCGAGAGTACCAATGGCTCCGACCCG-3′ and 5′-TTGGAGATCTTTCAGCAGTAGTCAGGGGAAC-3′. The PCR product obtained was cloned into the XhoI and BglII sites of pSP64TXB.

For injection, linearize with BamH1 and transcribe with SP6.

DNA (Catalog # 16857-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 16857-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.