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XE133 dnWnt-1 myc CS2+
(Plasmid #16859)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16859 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dnWnt-1 myc CS2+
  • Alt name
    wnt1
  • Species
    M. musculus (mouse)
  • Mutation
    Wnt-1 gene is truncated after the SPNFC peptide sequence
  • Entrez Gene
    Wnt1 (a.k.a. Int, Int-1, Wnt-, Wnt-1, sw, swaying)
  • Tag / Fusion Protein
    • myc

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Transcription: For sense, linearize with Asp718 (BMB) and transcribe with.
SP6. PCR amplified from myc tagged mouse Wnt-1 (McMahon and Moon). Also.
referred to as int-1 spnfc or int-1 spnfcD in notebook #3. The Wnt-1 gene.
is truncated acter the SPNFC peptide sequence.

Notes for Use:
Constructed by J. Brown.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE133 dnWnt-1 myc CS2+ was a gift from Randall Moon (Addgene plasmid # 16859 ; http://n2t.net/addgene:16859 ; RRID:Addgene_16859)