XE133 dnWnt-1 myc CS2+
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16859||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5010
Vector typeXenopus Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namednWnt-1 myc CS2+
SpeciesM. musculus (mouse)
MutationWnt-1 gene is truncated after the SPNFC peptide sequence
Entrez GeneWnt1 (a.k.a. Int, Int-1, Wnt-, Wnt-1, sw, swaying)
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Transcription: For sense, linearize with Asp718 (BMB) and transcribe with.
SP6. PCR amplified from myc tagged mouse Wnt-1 (McMahon and Moon). Also.
referred to as int-1 spnfc or int-1 spnfcD in notebook #3. The Wnt-1 gene.
is truncated acter the SPNFC peptide sequence.
Notes for Use:
Constructed by J. Brown.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE133 dnWnt-1 myc CS2+ was a gift from Randall Moon (Addgene plasmid # 16859 ; http://n2t.net/addgene:16859 ; RRID:Addgene_16859)