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pHIV-Wnt1HA
(Plasmid #18983)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18983 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    HIV-Zsgreen
  • Backbone size w/o insert (bp) 7664
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Growth in Stbl3 is preferred
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    wingless-related MMTV integration site 1
  • Alt name
    Wnt1
  • Alt name
    Mouse int-1 proto-oncogene
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1100
  • GenBank ID
    M11943
  • Entrez Gene
    Wnt1 (a.k.a. Int, Int-1, Wnt-, Wnt-1, sw, swaying)
  • Tag / Fusion Protein
    • HA-Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer 5’-TGGAATTTGCCCTTTTTGAG-3’
  • 3′ sequencing primer 5’-AGGAACTGCTTCCTTCACGA-3’
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Fung,Y.K., Shackleford,G.M., Brown,A.M., Sanders,G.S. and Varmus,H.E. Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene. Mol. Cell. Biol. 5 (12), 3337-3344 (1985)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

The Wnt1HA insert was cloned into the XbaI site of HIV-Zsgreen in order to generate a bicistronic lentivirus that coexpresses Wnt1HA with Zsgreen under the control of the EF1alpha promoter. Gerrit Dijkgraaf in Zena Werb's lab cloned this virus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIV-Wnt1HA was a gift from Bryan Welm & Zena Werb (Addgene plasmid # 18983 ; http://n2t.net/addgene:18983 ; RRID:Addgene_18983)
  • For your References section:

    Lentiviral transduction of mammary stem cells for analysis of gene function during development and cancer. Welm BE, Dijkgraaf GJ, Bledau AS, Welm AL, Werb Z. Cell Stem Cell. 2008 Jan 10. 2(1):90-102. 10.1016/j.stem.2007.10.002 PubMed 18371425