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ZE35 Nlk1myc-pCS2+
(Plasmid #17076)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 17076 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Alt name
    nemo-like kinase
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    nlk1 (a.k.a. nlk, nlkb, zgc:111875)
  • Tag / Fusion Protein
    • myc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert.
Epitope tag of a single c-myc tag cloned at the C-terminus of the NLK.

Species of Sequence Origin: zebrafish.

Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.

Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE35 Nlk1myc-pCS2+ was a gift from Randall Moon (Addgene plasmid # 17076 ; ; RRID:Addgene_17076)