|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17077||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeZebrafish Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt namenemo-like kinase
SpeciesD. rerio (zebrafish)
Insert Size (bp)1425
MutationC387Y. Inactive mutant of NLK1.
Entrez Genenlk1 (a.k.a. nlk, nlkb, zgc:111875)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert.
Clone is an inactive mutant of NLK1, analogous to C425Y mutant of mouse NLK which does not bind TCFs.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.
Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE36 Nlk1(C387Y)-pCS2+ was a gift from Randall Moon (Addgene plasmid # 17077 ; http://n2t.net/addgene:17077 ; RRID:Addgene_17077)