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Addgene

pmRuby2-CrowdingLinker-Clover
(Plasmid #171058)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171058 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pClover-mRuby2-FRET-10
  • Backbone manufacturer
    Michael Davidson
  • Backbone size w/o insert (bp) 5395
  • Total vector size (bp) 5758
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Crowdedness-sensitive linker domain
  • Species
    Synthetic
  • Insert Size (bp)
    300
  • Promoter CMV
  • Tags / Fusion Proteins
    • mRuby2 (N terminal on insert)
    • mClover2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmRuby2-CrowdingLinker-Clover was a gift from Ke Xu (Addgene plasmid # 171058 ; http://n2t.net/addgene:171058 ; RRID:Addgene_171058)
  • For your References section:

    Excitation spectral microscopy for highly multiplexed fluorescence imaging and quantitative biosensing. Chen K, Yan R, Xiang L, Xu K. Light Sci Appl. 2021 May 8;10(1):97. doi: 10.1038/s41377-021-00536-3. 10.1038/s41377-021-00536-3 PubMed 33963178