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pCIneo-AR-S81E
(Plasmid #171224)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 171224 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCIneo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human androgen receptor Ser81Glu mutant
  • Alt name
    AR-S81E
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Mutation
    AR-Ser81Glu (AR-S81E)
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV_fwd_primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Mutation in an AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIneo-AR-S81E was a gift from Steven Balk (Addgene plasmid # 171224 ; http://n2t.net/addgene:171224 ; RRID:Addgene_171224)
  • For your References section:

    Androgen receptor phosphorylation and stabilization in prostate cancer by cyclin-dependent kinase 1. Chen S, Xu Y, Yuan X, Bubley GJ, Balk SP. Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15969-74. doi: 10.1073/pnas.0604193103. Epub 2006 Oct 16. 10.1073/pnas.0604193103 PubMed 17043241