AID'-BE4max
(Plasmid
#174696)
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PurposeBase Editing
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174696 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAID'-BE4max
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SpeciesH. sapiens (human)
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MutationUGI-E11K
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Entrez GeneAICDA (a.k.a. AID, ARP2, CDA2, HEL-S-284, HIGM2)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' Cloning Site: NotI (unknown if destroyed), 3' Cloning Site: XmaI (unknown if destroyed).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AID'-BE4max was a gift from Rahul Kohli (Addgene plasmid # 174696 ; http://n2t.net/addgene:174696 ; RRID:Addgene_174696) -
For your References section:
Controllable genome editing with split-engineered base editors. Berrios KN, Evitt NH, DeWeerd RA, Ren D, Luo M, Barka A, Wang T, Bartman CR, Lan Y, Green AM, Shi J, Kohli RM. Nat Chem Biol. 2021 Dec;17(12):1262-1270. doi: 10.1038/s41589-021-00880-w. Epub 2021 Oct 18. 10.1038/s41589-021-00880-w PubMed 34663942
