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pCS2-BiNLS-fYap
(Plasmid #176623)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 176623 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4091
  • Total vector size (bp) 6551
  • Vector type
    Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Yes associated protein
  • Alt name
    Yap
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2706
  • Entrez Gene
    yap1 (a.k.a. YAP65, cb194, sb:cb194, si:ch211-181p1.5, si:dkey-3b8.3, wu:fc18c04, zYAP, zgc:158380)
  • Promoter SP6
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site PspXI (unknown if destroyed)
  • 5′ sequencing primer ATTTAGGTGACACTATAGA
  • 3′ sequencing primer ACTATAGTGAGTCGTATTA
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Vector backbone pDN34 is based on the LINuS method for optogenetic control of nuclear import.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-BiNLS-fYap was a gift from Timothy Saunders (Addgene plasmid # 176623 ; http://n2t.net/addgene:176623 ; RRID:Addgene_176623)
  • For your References section:

    Optogenetic control of YAP cellular localisation and function. Toh PJY, Lai JKH, Hermann A, Destaing O, Sheetz MP, Sudol M, Saunders TE. EMBO Rep. 2022 Sep 5;23(9):e54401. doi: 10.15252/embr.202154401. Epub 2022 Jul 25. 10.15252/embr.202154401 PubMed 35876586