Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17666||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6400
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
In the Author's Map, plasmid pEVX contains the
EVX proviral DNA (outlined), flanking cellular DNA (bold black line), and
pBR322 DNA (narrow black line). The LTR's are Moloney murine leukemia virus (MoMLV) long terminal repeats (LTRs).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEVX was a gift from Michael Botchan (Addgene plasmid # 17666 ; http://n2t.net/addgene:17666 ; RRID:Addgene_17666)
For your References section:Transformation mediated by the SV40 T antigens: separation of the overlapping SV40 early genes with a retroviral vector. Kriegler M, Perez CF, Hardy C, Botchan M. Cell. 1984 Sep . 38(2):483-91. 10.1016/0092-8674(84)90503-8 PubMed 6088084
Map uploaded by the depositor.