IFNGR2_pcDNA6.2/EmGFP-Bsd
(Plasmid #176939)

• Purpose: Mammalian expression vector encoding IFNGR2 and EmGFP-Bsd
• Depositing Lab: Roger Reeves
• Publication: Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712. Epub 2023 Apr 13.

Backbone

• Vector backbone: pcDNA6.2/EmGFP-Bsd/V5-DEST
• Backbone manufacturer: Invitrogen (V36620)
• Backbone size w/o insert (bp): 5983
• Vector type: Mammalian Expression
• Selectable markers: EmGFP fused to Blasticidin (bsd)-resistance marker

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IFNGR2
• Alt name: AF-1
• Alt name: IFGR2
• Species: H. sapiens (human)
• Insert Size (bp): 1014
• Entrez Gene: IFNGR2 (a.k.a. AF-1, IFGR2, IFNGT1, IMD28)
• Promoter: CMV

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: V5 reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Derived from Addgene #53823

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

IFNGR2 (NM_005534) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK