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(Plasmid #177336)


Item Catalog # Description Quantity Price (USD)
Plasmid 177336 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    kalium channelrhodopsin 1
  • Species
    Hyphochytrium catenoides
  • Insert Size (bp)
  • Mutation
    mammalian codon-adapted
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HcKCR1_mCherry_pcDNA3.1 was a gift from John Spudich (Addgene plasmid # 177336 ; ; RRID:Addgene_177336)
  • For your References section:

    Kalium channelrhodopsins are natural light-gated potassium channels that mediate optogenetic inhibition. Govorunova EG, Gou Y, Sineshchekov OA, Li H, Lu X, Wang Y, Brown LS, St-Pierre F, Xue M, Spudich JL. Nat Neurosci. 2022 Jun 20. pii: 10.1038/s41593-022-01094-6. doi: 10.1038/s41593-022-01094-6. 10.1038/s41593-022-01094-6 PubMed 35726059